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rabbit anti human semaphorin4d antibody  (Bioss)


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    Bioss rabbit anti human semaphorin4d antibody
    Transcript analysis and immunohistochemistry of Semaphorins and Netrins. ( a , b ) Elastin staining (Verhoeff-Van Gieson) and degradation score in non-diseased and TAA sections of size as indicated. Dashed boxes indicate magnified areas. Scale bars represent 1000 µm and 200 µm in low and high magnified images. Arrow indicate broken elastin fragments and stars indicate areas of complete elastin degradation. *** p < 0.001. Non-diseased ( n = 4), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5) ( c ) Quantitative PCR analysis of <t>Semaphorin4D</t> transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( d ) Hematoxylin and Eosin (H & E) image and representative immunofluorescence staining of Semaphorin4D (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( e ) Quantification of Semaphorin4D immunofluorescence staining in non-diseased and TAA. ( f ) Quantitative PCR analysis of PlexinB1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( g ) H & E image and representative fluorescence staining of PlexinB1 (red) and Dapi (blue) in non-diseased and TAA. ( h ) Quantification of PlexinB1 immunofluorescence staining in non-diseased and TAA. ( i ) Quantitative PCR analysis of Netrin-1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( j ) H & E image and representative immunofluorescence staining of Netrin-1 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( k ) Quantification of Netrin-1 immunofluorescence staining in non-diseased and TAA. ( l ) Quantitative PCR analysis of Netrin-3 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( m ) H & E image and representative immunofluorescence staining of Netrin-3 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining. ( n ) Quantification of Netrin-3 immunofluorescence staining in non-diseased and TAA. * p < 0.05 ** p < 0.01 *** p <0.001. L = lumen. Scale bars represent 500 µm on H & E images. Magnified scale bars are 50 µm.
    Rabbit Anti Human Semaphorin4d Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+semaphorin4d+antibody/pmc06539328-168-21-26?v=Bioss
    Average 90 stars, based on 1 article reviews
    rabbit anti human semaphorin4d antibody - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Mapping Semaphorins and Netrins in the Pathogenesis of Human Thoracic Aortic Aneurysms"

    Article Title: Mapping Semaphorins and Netrins in the Pathogenesis of Human Thoracic Aortic Aneurysms

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20092100

    Transcript analysis and immunohistochemistry of Semaphorins and Netrins. ( a , b ) Elastin staining (Verhoeff-Van Gieson) and degradation score in non-diseased and TAA sections of size as indicated. Dashed boxes indicate magnified areas. Scale bars represent 1000 µm and 200 µm in low and high magnified images. Arrow indicate broken elastin fragments and stars indicate areas of complete elastin degradation. *** p < 0.001. Non-diseased ( n = 4), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5) ( c ) Quantitative PCR analysis of Semaphorin4D transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( d ) Hematoxylin and Eosin (H & E) image and representative immunofluorescence staining of Semaphorin4D (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( e ) Quantification of Semaphorin4D immunofluorescence staining in non-diseased and TAA. ( f ) Quantitative PCR analysis of PlexinB1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( g ) H & E image and representative fluorescence staining of PlexinB1 (red) and Dapi (blue) in non-diseased and TAA. ( h ) Quantification of PlexinB1 immunofluorescence staining in non-diseased and TAA. ( i ) Quantitative PCR analysis of Netrin-1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( j ) H & E image and representative immunofluorescence staining of Netrin-1 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( k ) Quantification of Netrin-1 immunofluorescence staining in non-diseased and TAA. ( l ) Quantitative PCR analysis of Netrin-3 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( m ) H & E image and representative immunofluorescence staining of Netrin-3 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining. ( n ) Quantification of Netrin-3 immunofluorescence staining in non-diseased and TAA. * p < 0.05 ** p < 0.01 *** p <0.001. L = lumen. Scale bars represent 500 µm on H & E images. Magnified scale bars are 50 µm.
    Figure Legend Snippet: Transcript analysis and immunohistochemistry of Semaphorins and Netrins. ( a , b ) Elastin staining (Verhoeff-Van Gieson) and degradation score in non-diseased and TAA sections of size as indicated. Dashed boxes indicate magnified areas. Scale bars represent 1000 µm and 200 µm in low and high magnified images. Arrow indicate broken elastin fragments and stars indicate areas of complete elastin degradation. *** p < 0.001. Non-diseased ( n = 4), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5) ( c ) Quantitative PCR analysis of Semaphorin4D transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( d ) Hematoxylin and Eosin (H & E) image and representative immunofluorescence staining of Semaphorin4D (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( e ) Quantification of Semaphorin4D immunofluorescence staining in non-diseased and TAA. ( f ) Quantitative PCR analysis of PlexinB1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( g ) H & E image and representative fluorescence staining of PlexinB1 (red) and Dapi (blue) in non-diseased and TAA. ( h ) Quantification of PlexinB1 immunofluorescence staining in non-diseased and TAA. ( i ) Quantitative PCR analysis of Netrin-1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( j ) H & E image and representative immunofluorescence staining of Netrin-1 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( k ) Quantification of Netrin-1 immunofluorescence staining in non-diseased and TAA. ( l ) Quantitative PCR analysis of Netrin-3 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( m ) H & E image and representative immunofluorescence staining of Netrin-3 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining. ( n ) Quantification of Netrin-3 immunofluorescence staining in non-diseased and TAA. * p < 0.05 ** p < 0.01 *** p <0.001. L = lumen. Scale bars represent 500 µm on H & E images. Magnified scale bars are 50 µm.

    Techniques Used: Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction, Immunofluorescence, Fluorescence



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    rabbit anti human semaphorin4d antibody  (Bioss)
    90
    Bioss rabbit anti human semaphorin4d antibody
    Transcript analysis and immunohistochemistry of Semaphorins and Netrins. ( a , b ) Elastin staining (Verhoeff-Van Gieson) and degradation score in non-diseased and TAA sections of size as indicated. Dashed boxes indicate magnified areas. Scale bars represent 1000 µm and 200 µm in low and high magnified images. Arrow indicate broken elastin fragments and stars indicate areas of complete elastin degradation. *** p < 0.001. Non-diseased ( n = 4), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5) ( c ) Quantitative PCR analysis of <t>Semaphorin4D</t> transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( d ) Hematoxylin and Eosin (H & E) image and representative immunofluorescence staining of Semaphorin4D (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( e ) Quantification of Semaphorin4D immunofluorescence staining in non-diseased and TAA. ( f ) Quantitative PCR analysis of PlexinB1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( g ) H & E image and representative fluorescence staining of PlexinB1 (red) and Dapi (blue) in non-diseased and TAA. ( h ) Quantification of PlexinB1 immunofluorescence staining in non-diseased and TAA. ( i ) Quantitative PCR analysis of Netrin-1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( j ) H & E image and representative immunofluorescence staining of Netrin-1 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( k ) Quantification of Netrin-1 immunofluorescence staining in non-diseased and TAA. ( l ) Quantitative PCR analysis of Netrin-3 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( m ) H & E image and representative immunofluorescence staining of Netrin-3 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining. ( n ) Quantification of Netrin-3 immunofluorescence staining in non-diseased and TAA. * p < 0.05 ** p < 0.01 *** p <0.001. L = lumen. Scale bars represent 500 µm on H & E images. Magnified scale bars are 50 µm.
    Rabbit Anti Human Semaphorin4d Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+semaphorin4d+antibody/pmc06539328-168-21-26?v=Bioss
    Average 90 stars, based on 1 article reviews
    rabbit anti human semaphorin4d antibody - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    Transcript analysis and immunohistochemistry of Semaphorins and Netrins. ( a , b ) Elastin staining (Verhoeff-Van Gieson) and degradation score in non-diseased and TAA sections of size as indicated. Dashed boxes indicate magnified areas. Scale bars represent 1000 µm and 200 µm in low and high magnified images. Arrow indicate broken elastin fragments and stars indicate areas of complete elastin degradation. *** p < 0.001. Non-diseased ( n = 4), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5) ( c ) Quantitative PCR analysis of Semaphorin4D transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( d ) Hematoxylin and Eosin (H & E) image and representative immunofluorescence staining of Semaphorin4D (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( e ) Quantification of Semaphorin4D immunofluorescence staining in non-diseased and TAA. ( f ) Quantitative PCR analysis of PlexinB1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( g ) H & E image and representative fluorescence staining of PlexinB1 (red) and Dapi (blue) in non-diseased and TAA. ( h ) Quantification of PlexinB1 immunofluorescence staining in non-diseased and TAA. ( i ) Quantitative PCR analysis of Netrin-1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( j ) H & E image and representative immunofluorescence staining of Netrin-1 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( k ) Quantification of Netrin-1 immunofluorescence staining in non-diseased and TAA. ( l ) Quantitative PCR analysis of Netrin-3 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( m ) H & E image and representative immunofluorescence staining of Netrin-3 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining. ( n ) Quantification of Netrin-3 immunofluorescence staining in non-diseased and TAA. * p < 0.05 ** p < 0.01 *** p <0.001. L = lumen. Scale bars represent 500 µm on H & E images. Magnified scale bars are 50 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Mapping Semaphorins and Netrins in the Pathogenesis of Human Thoracic Aortic Aneurysms

    doi: 10.3390/ijms20092100

    Figure Lengend Snippet: Transcript analysis and immunohistochemistry of Semaphorins and Netrins. ( a , b ) Elastin staining (Verhoeff-Van Gieson) and degradation score in non-diseased and TAA sections of size as indicated. Dashed boxes indicate magnified areas. Scale bars represent 1000 µm and 200 µm in low and high magnified images. Arrow indicate broken elastin fragments and stars indicate areas of complete elastin degradation. *** p < 0.001. Non-diseased ( n = 4), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5) ( c ) Quantitative PCR analysis of Semaphorin4D transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( d ) Hematoxylin and Eosin (H & E) image and representative immunofluorescence staining of Semaphorin4D (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( e ) Quantification of Semaphorin4D immunofluorescence staining in non-diseased and TAA. ( f ) Quantitative PCR analysis of PlexinB1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 6). ( g ) H & E image and representative fluorescence staining of PlexinB1 (red) and Dapi (blue) in non-diseased and TAA. ( h ) Quantification of PlexinB1 immunofluorescence staining in non-diseased and TAA. ( i ) Quantitative PCR analysis of Netrin-1 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( j ) H & E image and representative immunofluorescence staining of Netrin-1 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining ( k ) Quantification of Netrin-1 immunofluorescence staining in non-diseased and TAA. ( l ) Quantitative PCR analysis of Netrin-3 transcripts in non-diseased ( n = 3), TAA < 5.5 cm ( n = 4), TAA > 5.5 cm ( n = 5). ( m ) H & E image and representative immunofluorescence staining of Netrin-3 (red) and Dapi (blue) in non-diseased and TAA sections of human aorta. Dashed boxes indicate magnified areas of immunofluorescence staining. ( n ) Quantification of Netrin-3 immunofluorescence staining in non-diseased and TAA. * p < 0.05 ** p < 0.01 *** p <0.001. L = lumen. Scale bars represent 500 µm on H & E images. Magnified scale bars are 50 µm.

    Article Snippet: Following is the list of primary and secondary antibodies that were used: Mouse anti-human Semaphorin4D antibody (ab212275, Abcam, Cambridge, MA, USA), Rabbit anti-human Semaphorin4D antibody (bs-6965R, Bioss antibodies, Woburn, MA, USA), Rabbit anti-human PlexinB1 antibody (ab90087, Abcam), Rabbit anti-human Netrin-1 antibody (ab126729, Abcam), Rabbit anti-human Netrin-3 antibody (bs-11059R, Bioss antibodies, Woburn, MA, USA), Goat anti-rabbit antibody Alexa Fluor 568 (A11011, Invitrogen, Wilmington, DE, USA), Goat anti-mouse antibody Alexa Fluor 568 (S11004, Invitrogen), 4′,6-diamidino-2-phénylindole (Dapi, 1: 50,000 dilution; D1306, Invitrogen).

    Techniques: Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction, Immunofluorescence, Fluorescence